Evaluation of the Antifungal Activity of Endophytic and Rhizospheric Bacteria against Grapevine Trunk Pathogens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Isolation of Bacterial Endophytes
2.2. Initial Screening of Grapevine Endophytic and Rhizospheric Bacteria for Antifungal Activity against Neofusicoccum parvum and Diplodia seriata
2.3. Molecular Identification of Bacterial Isolates
2.4. Dual Antagonism Assays of Selected Bacterial Isolates against Grapevine Trunk Pathogens
2.5. Effect of Bacterial Agar-Diffusible Metabolites on Grapevine Trunk Pathogens
2.6. Effect of Bacterial Volatile Organic Compounds (VOCs) on Grapevine Trunk Pathogens
2.7. Statistical Analyses
3. Results
3.1. Initial Screening of Grapevine Endophytic and Rhizospheric Bacteria for Antifungal Activity against Neofusicoccum parvum and Diplodia seriata
3.2. Dual Antagonism Assays of Selected Bacterial Isolates against Grapevine Trunk Pathogens
3.3. Effect of Bacterial Agar-Diffusible Metabolites against Grapevine Trunk Pathogens
3.4. Effect of Bacterial Volatile Organic Compounds on Grapevine Trunk Pathogens
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bacterial Species | Isolate | Tissue | Vine Health Status | County | Cultivar |
---|---|---|---|---|---|
Bacillus velezensis | UCD10614 | Cordon | Asymptomatic | Santa Barbara | Pinot Noir |
UCD10631 | Trunk | Asymptomatic | San Luis Obispo | Cabernet Sauvignon | |
Pseudomonas chlororaphis | UCD10757 | Rhizosphere | Symptomatic | Monterey | Chardonnay |
UCD10763 | Rhizosphere | Asymptomatic | Riverside | Scarlet Royal | |
Serratia plymuthica | UCD10719 | Rhizosphere | Asymptomatic | Fresno | Thompson Seedless |
UCD10756 | Rhizosphere | Asymptomatic | Tulare | Thompson Seedless |
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Bustamante, M.I.; Elfar, K.; Eskalen, A. Evaluation of the Antifungal Activity of Endophytic and Rhizospheric Bacteria against Grapevine Trunk Pathogens. Microorganisms 2022, 10, 2035. https://doi.org/10.3390/microorganisms10102035
Bustamante MI, Elfar K, Eskalen A. Evaluation of the Antifungal Activity of Endophytic and Rhizospheric Bacteria against Grapevine Trunk Pathogens. Microorganisms. 2022; 10(10):2035. https://doi.org/10.3390/microorganisms10102035
Chicago/Turabian StyleBustamante, Marcelo I., Karina Elfar, and Akif Eskalen. 2022. "Evaluation of the Antifungal Activity of Endophytic and Rhizospheric Bacteria against Grapevine Trunk Pathogens" Microorganisms 10, no. 10: 2035. https://doi.org/10.3390/microorganisms10102035