Fungi Associated with Postharvest Diseases of Sweet Potato Storage Roots and In Vitro Antagonistic Assay of Trichoderma harzianum against the Diseases
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolation
2.2. DNA Extraction, PCR Amplification, and Sequencing
2.3. Molecular Phylogeny
2.4. Morphology
2.5. Pathogenicity and Reisolation
2.6. In Vitro Biocontrol Activity
3. Results
3.1. Associated Pathogenic Fungi
3.2. Molecular Phylogeny
3.3. Morphology
3.4. Taxonomy: Aspergillus wentii CMML21–4
3.5. Taxonomy: Penicillium rotoruae CMML21–7
3.6. Pathogenicity
3.7. In Vitro Antifungal Activity
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Location | Number of Fungi Isolated | ||||
---|---|---|---|---|---|
Fusarium spp. | Penicillium spp. | Aspergillus sp. | Mucor sp. | Macrophomina sp. | |
Cheonan-si | 12 | 7 | 4 | – | – |
Haenam-gun | 24 | – | – | 1 | – |
Buan-gun | 10 | – | – | – | 10 |
No. of fungi | 46 | 7 | 4 | 1 | 10 |
Isolation (%) | (67.6%) | (10.3%) | (5.9%) | (1.5%) | (14.7%) |
Total | 68 |
Fungal Isolates | Disease Length (mm) | Disease Depth (mm) |
---|---|---|
Fusarium oxysporum CMML21–2 | 13.52 ± 0.95 c | 6.67 ± 1.92 b |
Aspergillus wentii CMML21–4 | 15.32 ± 0.61 c | 7.80 ± 0.95 b |
Penicillium citrinum CMML21–5 | 12.96 ± 1.02 c | 6.20 ± 0.66 b |
Penicillium rotoruae CMML21–7 | 15.55 ± 0.32 c | 7.23 ± 0.39 b |
Fusarium ipomoeae CMML21–8 | 23.47 ± 2.09 b | 9.30 ± 1.11 b |
Mucor variicolumellatus CMML21–12 | 12.65 ± 0.58 c | 5.03 ± 1.47 b |
Fusarium oxysporum CMML21–13 | 16.85 ± 2.22 bc | 9.27 ± 1.13 b |
Macrophomina phaseolina CMML21–16 | 112.95 ± 2.25 a | 42.80 ± 0.30 a |
Fusarium solani CMML21–17 | 14.0 6 ± 2.03 c | 6.90 ± 1.15 b |
Fungal Isolates | Inhibition Rate (%) | |
---|---|---|
CMML20–26 | CMML20–27 | |
Fusarium oxysporum CMML21–2 | 81.14 ± 3.07 a | 72.05 ± 0.92 ab |
Aspergillus wentii CMML21–4 | 77.04 ± 0.69 abc | 73.93 ± 2.50 a |
Penicillium citrinum CMML21–5 | 53.87 ± 2.04 d | 53.87 ± 0.48 de |
Penicillium rotoruae CMML21–7 | 69.40 ± 0.51 bc | 58.13 ± 1.35 cd |
Fusarium ipomoea CMML21–8 | 56.99 ± 3.44 d | 57.06 ± 1.60 cd |
Mucorvariicolumellatus CMML21–12 | 50.21 ± 1.27 d | 42.66 ± 1.28 e |
Fusarium oxysporum CMML21–13 | 75.29 ± 0.49 abc | 72.88 ± 2.99 ab |
Macrophominaphaseolina CMML21–16 | 78.22 ± 0.91 ab | 75.01 ± 2.50 a |
Fusarium solani CMML21–17 | 68.83 ± 0.89 c | 64.94 ± 0.65 bc |
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Paul, N.C.; Park, S.; Liu, H.; Lee, J.G.; Han, G.H.; Kim, H.; Sang, H. Fungi Associated with Postharvest Diseases of Sweet Potato Storage Roots and In Vitro Antagonistic Assay of Trichoderma harzianum against the Diseases. J. Fungi 2021, 7, 927. https://doi.org/10.3390/jof7110927
Paul NC, Park S, Liu H, Lee JG, Han GH, Kim H, Sang H. Fungi Associated with Postharvest Diseases of Sweet Potato Storage Roots and In Vitro Antagonistic Assay of Trichoderma harzianum against the Diseases. Journal of Fungi. 2021; 7(11):927. https://doi.org/10.3390/jof7110927
Chicago/Turabian StylePaul, Narayan Chandra, Soyoon Park, Haifeng Liu, Ju Gyeong Lee, Gui Hwan Han, Hyunsook Kim, and Hyunkyu Sang. 2021. "Fungi Associated with Postharvest Diseases of Sweet Potato Storage Roots and In Vitro Antagonistic Assay of Trichoderma harzianum against the Diseases" Journal of Fungi 7, no. 11: 927. https://doi.org/10.3390/jof7110927