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RF2JKMDKM–Female scientist working in modern lab. Doctor making microbiology research. Laboratory tools: microscope, test tubes, equipment. Coronavirus covid-19, bacteriology, virology, dna and health care.
RF2HCD6WB–Laboratory pipette filled with blue liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Blue liquid in pe
RMPFTPYE–. A laboratory manual of soil bacteriology. Soil microbiology. SPECIAL METHODS 159 necessary. Although there are a great number of vessels designed for this purpose, only one will be described. It is obvious from the start that apparatus of this nature must be somewhat complicated. A modification of the Schulze and Schulow methods has been found fairly satis- factory. Apparatus: One large Woulfe's bottle, about 3-liter capacity, with three openings. One large 2- or 3-litcr flask, Erlcnmeyer form. Two U tubes. One cylinder constructed as shown in Fig. 13. The apparatus is arranged according to
RM2AWW6G6–Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . e loop-ful of an eighteen-hour-old agar culture to i c.c. salt solution),and enough of immune serum and salt solution to make thenecessary dilution and up to 20 drops. A series of controlsis made with normal serum and the same amount of microbicculture and guinea-pig serum. After eighteen hours the cholera vibrios will be active in thecontrol, but dissolved and clumped up in the tubes containingthe immune serum. CHAPTER XXnBACTEMA IN PNEUMONIA Klebs in 1875 called atten
RFFW8JFH–closeup of a young caucasian man in white coat with test tubes with liquids of different colors in a laboratory
RF2AXE32H–Laboratory assistant analyzes bacterium sample using medical equipment in a bacteriology clinic. Microbiological medical concept
RF2JKMDF0–Female scientist working in modern lab. Doctor making microbiology research. Laboratory tools: microscope, test tubes, equipment. Coronavirus covid-19, bacteriology, virology, dna and health care.
RF2HCD531–Laboratory pipette filled with red liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Red liquid in the
RMPFTPC4–. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. D Petri Dishes. These should be about 10 cm. in diameter, thus having an area of about 65 sq. cm. Either glass or porous tops may be used. The porous tops devised by Hill prevent the spreading colonies which appear so often on Petri dishes in humid weather. Very often it is difficult to secure Petri dishes with flat bottoms. This is essential to an even distribution of bacteria over the plate and a unif
RM2AJGF43–Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . extend into the ves-icular structures, giv-ing rise to lobularpneumonia. In thelarger tubes theglands of the mucosamay stand out prom-inently, constitutinga follicular bronchitis.When the inflammatory products are abundant, clear, serous fluids,the name bronchorrhea is given; bronchoblennorrhea is applied to thecondition when a puriform expectoration is present. These probablyrepresent different stages in the same inflammatory process. Putridor fetid bronchitis is due to decomposition
RFFRP3EA–closeup of a young caucasian man in white coat with a dropper in his hand and some test tubes with liquids of different colors i
RF2AXE32D–Laboratory assistant analyzes bacterium sample using medical equipment in a bacteriology clinic. Microbiological medical concept
RF2JKMDH6–Female scientist working in modern lab. Doctor making microbiology research. Laboratory tools: microscope, test tubes, equipment. Coronavirus covid-19, bacteriology, virology, dna and health care.
RF2HCD55E–Laboratory pipette filled with red liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Red liquid in the
RMPFTPPG–. Bacteria in relation to soil fertility. Soil microbiology; Bacteriology, Agricultural. THE CHEMISTRY OF BACTERIA 43 required for the growth of different bacteria just as various higher plants require different soils. Most bacteria grow well in beef tea. However, we must prevent the organisms from swim- ming about and thus mixing during growth. This is accom- plished by adding to the medium one per cent of a seaweed, agar- agar. Such a medium is placed in large flasks, in test tubes stop- pered with cotton plugs, or shallow dishes covered with a second. Fig. 21.—Sterilizers used in bacteriolo
RM2AWWYR7–Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . The tubes can be placed directly under the microscope forfurther examination of the colonies. Animals as Culture-media.—It isalmost impossible to separate certainorganisms, such as the tubercle bacil-lus and pneumococcus, from mixedcultures by ordinary plate methods,and the plan of producing the diseasein animals by inoculation, and thenobtaining the organism in pure cul-ture, has to be employed. Pure Cultures by Boiling.—Spored organisms may be separatedfrom others by
RF2AXE329–Laboratory assistant analyzes bacterium sample using medical equipment in a bacteriology clinic. Microbiological medical concept
RF2JKMBY3–Male scientist working in modern lab. Doctor making microbiology research. Laboratory tools: microscope, test tubes, equipment. Coronavirus covid-19, bacteriology, virology, dna and health car
RF2HCD70G–Laboratory pipette filled with blue liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Blue liquid in pe
RMPG2R41–. Laboratory work in bacteriology. Bacteriology. 244 BACTEBIOLOGY. der to obtain results. The apparatus employed for this purpose is known as an incubator or thermostat. It is made of heavy copper and has double walls. The space between these, which should be as large as possible, is filled with distilled water. In form the thermostat may be oblong, square or oval. Pig. 36 shows an excellent thermostat of oval form.. Fig. 36. The incubator or thermostat. It is desirable that the incubator should have as much available space as possible for dishes, flasks, tubes, etc. The apparatus as ordinaril
RM2AJGFY9–Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . involve the structuresnamed. Perichondritis is, as itsname indicates, an inflam-mation of the perichon-drium. It is usuallysecondary to acute inflam-mations of the overlyingmucosa; less frequently itresults from extension ofan infectious process fromthe contiguous tissues.The condition is rarely-primary, commonly follow-ing tuberculosis, syphilis,and the more acute infec-tious diseases, such as ty-phoid and typhus fevers,smallpox, etc. Tubes leftin the trachea, probablyby continuous i
RF2AXE31C–Laboratory assistant analyzes bacterium sample using medical equipment in a bacteriology clinic. Microbiological medical concept
RF2JKM96T–Male arab scientist working in modern lab. Doctor making microbiology research. Laboratory tools: microscope, test tubes, equipment. Coronavirus covid-19, bacteriology, virology, dna and health care.
RF2HCD556–Laboratory pipette filled with red liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Red liquid in the
RMPFXBKF–. Bacteriology and mycology of foods. Food. 20 BACTERIOLOGICAL APPARATUS Smillie's Method. This method depends upon the catalytic action of platinized asbestos upon oxygen and hydrogen when they are brought into contact. The author claims that the method is well adapted to the study of strict anaerobes such as B, botuhnus. The method adapt- able to cultures in test tubes is described as follows by the author: *' Platinized asbestos is first prepared in the usual way, or it may be purchased from any laboratory supply house. A small mass of the catalyzer is firmly fixed at the end of a platinum
RM2AKKRK6–Practical dairy bacteriology, prepared for the use of students, dairymen, and all interested in the problems of the relation of milk to public health . orbent cotton is used for a strainer, it must be thrownaway after the milking. Milking Machines.—A still more recent means of reducingcontamination is by machines designed to convey the milkdirectly from the cows teat to closed sterilized milk cans, with-out allowing it to come into contact with the air, or exposingit to contamination with dirt.^ These milking machines consistof rubber tubes ending in special cups for attachment to theteats of
RF2HCD6XX–Laboratory pipette filled with blue liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Blue liquid in pe
RMPG1R1X–. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. Fig. 143. Tulostotna ntammosum, FlG. 144. Secotiumerythrecephalum, Fr. Basidia with fully formed spores TuL Sporophore divided in half^ of highly magnified. After Schrcjter. the natural size. After Tulasne. the constituents of the hymenium; and stouter tubes usually non-septate, which are members or branches of the same hyphae as the delicate elements and run for the most part in the trama, but may also, as in Lycoperdon and Bovista, send branches transversely through
RM2AJF7BA–Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . glass tubes. The liquefied medium is pouredinto the percolator; the test-tubes are then arrangedin the baskets at the side of the percolator. A test-tube or flask is grasped by the left hand, preferablyby the thumb and index-finger; the cotton is rotatedand loosened by means of the thumb and index-finger of the right hand, and removed by graspingthe projecting tip between the ring- and little fingers of the righthand; the tube is now carefully slipped over the filling tube from theper
RF2HCD765–Laboratory pipette filled with blue liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Blue liquid in pe
![. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. CHAPTER III.—SPORES OF FUNGI.—GERMINATION. "3 becomes invested with a very delicate membrane of its own and appears as a small vesicle which elongates in the outward direction as the germ-tube and grows through the episporium. In the thick-walled spores of Pertusaria the tubes often ramify inside the episporium and the ramifications spread in it parallel with the surface of the spore. The canals in the membrane are, as far as can be ascertained, new formations at Stock Photo](https://c8.alamy.com/comp/PG4387/comparative-morphology-and-biology-of-the-fungi-mycetozoa-and-bacteria-plant-morphology-fungi-myxomycetes-bacteriology-chapter-iiispores-of-fungigermination-quot3-becomes-invested-with-a-very-delicate-membrane-of-its-own-and-appears-as-a-small-vesicle-which-elongates-in-the-outward-direction-as-the-germ-tube-and-grows-through-the-episporium-in-the-thick-walled-spores-of-pertusaria-the-tubes-often-ramify-inside-the-episporium-and-the-ramifications-spread-in-it-parallel-with-the-surface-of-the-spore-the-canals-in-the-membrane-are-as-far-as-can-be-ascertained-new-formations-at-PG4387.jpg ". Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. CHAPTER III.—SPORES OF FUNGI.—GERMINATION. "3 becomes invested with a very delicate membrane of its own and appears as a small vesicle which elongates in the outward direction as the germ-tube and grows through the episporium. In the thick-walled spores of Pertusaria the tubes often ramify inside the episporium and the ramifications spread in it parallel with the surface of the spore. The canals in the membrane are, as far as can be ascertained, new formations at Stock Photo")
RMPG4387–. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. CHAPTER III.—SPORES OF FUNGI.—GERMINATION. "3 becomes invested with a very delicate membrane of its own and appears as a small vesicle which elongates in the outward direction as the germ-tube and grows through the episporium. In the thick-walled spores of Pertusaria the tubes often ramify inside the episporium and the ramifications spread in it parallel with the surface of the spore. The canals in the membrane are, as far as can be ascertained, new formations at
RM2AJF50G–Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . from which inoculations aremade at intervals of minutes for as long as an hour. The removal mustbe made into tubes containing sufficient of the medium to dilute thematerial carried over beyond the strength already shown to have aninhibitory action, so that any undestroyed bacteria may be free todevelop. The source of error by this method lies in the clumping of the germsin the original tube; if grouped in clumps, the agent may not havepenetrated the clumps. Penetration is a most impor
RF2HCD576–Laboratory pipette filled with red liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Red liquid in the
RMPG1TJE–. Laboratory work in bacteriology. Bacteriology. 516 BACTERIOLOGY. The contents of each of the capillary tube's are then in- oculated into bouillon which is set aside at 37° to allow the growth, it any, to develop. The contents are removed as follows: The tube is first wiped dry and then one end is slightly scratched with a file. The end is then removed and the opening of the tube is sterilized by touching it 2 or 3 times to a flame. The tube is held in a broad-pointed pair of forceps, and, when cool, the cut end is inserted into the culture tube while the closed end is slowly brought 'jnto co
RM2AG7G4E–. The principles of bacteriology: a practical manual for students and physicians. terilization complete. The tubes arethen removed and allowed to cool. The cotton used for this purpose should be the ordi-nary cotton batting of the shops, and not absorbentcotton; the latter becomes too tightly packed, and is,moreover, much too expensive for this purpose. Care should be taken not to burn the cotton, other-wise the tubes will become coated with a dark-colored,empyreumatic, oily deposit, which necessitates recleans-ing. Filling the Tubes.—When the tubes are coldthey may be filled. This is best acc
RF2HCD6T0–Laboratory pipette filled with blue liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Blue liquid in pe
RMPG442R–. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. 104 DIVISION I.âGENERAL MORPHOLOGY. mentioned also the envelope-cells on the spore-clusters of Urocystis, which will be described in Chapter V, but not the germ-tubes which appear inside the ascus in Sphaeria praecox, and which were described by Tulasne ^ as filiform appendages. We have already spoken, in concurrence with Zopf's views, of the physiological import of the gelatinous appendages as organs which may serve to attach the asco- spores to one another and to th
RM2AG7EBP–. The principles of bacteriology: a practical manual for students and physicians. h mayhave been introduced into the tubes. This is not ofso much moment with gelatin, since it may readily be TECHNIQUE OF MAKING PLATES. 133 redissolved at a temperature not detrimental to theorganisms with which the tubes may have been inocu-lated. The Cooling-stage and Levelling-teipod.—AVhile the medium of which the plates are to be madeis melting it is well to arrange the cooling-stage (Fig.24) upon which the gelatin or agar-agar is to be subse-quently solidified. This stage consists of a glass dish filled wi
RF2HCD767–Laboratory pipette filled with blue liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Blue liquid in pe
 and is thus independent of the regulat- ing parts. The upper portion consists of the three parts A, B and C, the arrangement and working of which is readily understood from the illustra- tion. The gas enters th Stock Photo")
RMPG2R38–. Laboratory work in bacteriology. Bacteriology. 246 BACTERIOLOGY. The author's thermo-regulator *, shown in Fig. 37, is intended to overcome the difficulties mentioned. Although, apparently, more complicated than the ordinary regulator, it nevertheless is easily adjusted and is extremely sensitive. The gas tubing is attached to the strong, wide inflow and outflow tubes {a' and a") and is thus independent of the regulat- ing parts. The upper portion consists of the three parts A, B and C, the arrangement and working of which is readily understood from the illustra- tion. The gas enters th
RM2AG7H40–. The principles of bacteriology: a practical manual for students and physicians. a sterilized pipette and placed in tall cylindersthat have previously been plugged with cotton waddingand sterilized. After treating all the serum in this way,care having been taken to exclude as much as possible of 112 BAaTEBIOLOGY. the coloring-matter of the blood, it may be placed againin the ice-chest for twenty-four hours, during whichtime the corpuscular elements will sink to the bottom,leaving the supernatant fluid quite clear. This maythen be pipetted off, either into sterilized test-tubes,about 8 c.c. to
RF2HCD75B–Laboratory pipette filled with blue liquid and resulting chemical solution on Petri dish for a biology experiment in his laboratory. Blue liquid in pe
RMPG4D0R–. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. 8a DIVISION I.—GENERAL MORPHOLOGV. and exceptional cases the release of the ripe spores is left to chance, there being no special arrangement made for it, and the spores may even germinate inside the mother- cell, the germ-tubes piercing or bursting through its wall, as may be seen in the sporangioles of Thamnidium and its allies. The arrangements for the escape of the spores vary in different species. a. The aquatic swarm-spores of the Saprolegnieae (with one partial
RM2AJ79FJ–Journal of bacteriology . ymeans of the switch which constitutes a part of the bath, and the desired temperature thus secured. The bath has a constant water level attachment, so that the water level may be kept constantat anv point within a range of 25 mm. The regularity of the water level is 1 mm. Capacity of bathICOO cc. As the heating units are inserted in brass tubes placed permanently inside of the baths, 20 mm.from the bottom, their heating value comes to 99% of the theoretical expenditure of energy. Placedas they are, the heating units are absolutely protected from accidental injury. To
![. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ] Stock Photo](https://c8.alamy.com/comp/PFTPC7/bacteriology-and-mycology-of-foods-food-4l-a-fig-1-types-of-fermentation-tubes-af-browns-j5-smiths-c-smiths-without-foot-d-graves-modification-of-the-durham-fermentation-tube-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-tanner-fred-wilbur-1888-1957-new-york-john-wiley-amp-sons-inc-etc-etc-PFTPC7.jpg ". Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ] Stock Photo")
RMPFTPC7–. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ]
RM2AG7D9R–. The principles of bacteriology: a practical manual for students and physicians. l for thiswork, as they do away with the necessity for the cool-ing-stage and levelling-tripod, though in warm weatherthe cooling-stage may be used to hasten the solidifica-tion of gelatin. A cooling-stage of very convenientdesign for use with these dishes consists of a closed, flatmetal box, either of copper or block tin, and roundor square in shape, so arranged that it can be filledwith cold water, or that cold water can constantly be ESMABOH TUBES. 137 passed through it by means of a rubber tube connectedwith
RMPG1TM0–. Laboratory work in bacteriology. Bacteriology. BLOOD-SERUM. 467 thermo-regulator is employed to secure a constant temperature. Heat is applied till a temperature of about 75° is reached and this is then maintained for 1 hour. This is repeated on each of three or four successive days.. The time necessary to secure sterilization can be shortened by transferring- the tubes, for a few hours after each heat, to an incubator at 37° as described in connection with the preceding method. Sterilization of serum at 100°.—Serum sterilized at this temperature is opaque white. This, howevei:, does not in-
RM2AWX23T–Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . -Flask toblood-se- NUTRIENT CULTUEE-MEDIA 73 having been placed in the thermostat, are kept at a temper-ature of 65° to 68° C. mi to. coagulation occurs; then removedand sterilized by fractional sterilization. Sterilization of Blood-serum.—^The tubes are placedthree to four days in incubator at 58° C, and those tubeswhich show any evidences of organic growth are discarded. If, now, at the end of a week, the serum remains sterile at. Fig. 21.—^Thermostat or inspissator f
RMPG1TJK–. Laboratory work in bacteriology. Bacteriology. 512 BACTERIOLOGY. The Sealing and Keeping of Cultures. The method of preserving cultures or specimens of blood, etc., in djawn-out bulb tubes has been touched upon (p. 458). In this connection it is desirable to indicate the methods employed in sealing the ordinary culture tubes. Three or four procedures are resorted to. The tubes may be sealed with rubber caps, corks, sealing-wax, or with parafln. Thin sheets of rubber may also be used. In either case, the cotton plug should be cut close to the end of the tube. It should then be drawn out sligh
RM2AJHNFX–Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . —The process arises almost exclusively in connection with tuberculosis, but is occasionally seen in other chronic infections; in some instances small nontuberculous abscesses may caseate. Caseation is sometimes seen in serous cavities: e. (^., pericardium or pleura. Rarely, Deut. Arch. f. klin. Med., 1905, Ixxxi, p. 163. NECROSIS. ^S5 the retention of mucoid materials, as in the Fallopian tubes, may befollowed by inspissation and caseation. 5. Sphacelation en masse, or Gangrene. This
RMPG2R5H–. Laboratory work in bacteriology. Bacteriology. 160 BACTERIOLOGY. dicated is of the author's own construction (see Fig. 37). As a rule, a regulator is not necessary when using the dry-heat sterilizer.. Fig. 25. Dry-heat sterilizer, double width. In the corners, perforated brass tubes to protect the thermometer and the thermoregulator. The time necessary to effect sterilization in the dry- heat oven will, of course, depend upon the temperature. The ordinary laboratory articles are quickly sterilized at 150°. When the thermometer indicates 150°, the time should be noted, and this temperature sh
RM2AJF7NP–Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . Fig. 425.—Instrument for Cutting Plugs of Potato forPotato Cultures. The cut plugs are washed in water and pushed down into test-tubes into which the potato segment fits closely. Enoughwater is added to come just up to the lower end of the plug.The tubes are then sterilized as directed in the text. Fig. 426.—Agate-ware Water-bath(Farina Kettle).Used in making culture media, and con-venient for many purposes about thelaboratory; the capacity should beabout 1000 c.c. for inside boiler.
RMPG2R4M–. Laboratory work in bacteriology. Bacteriology. GELATIN AND POTATO MEDIA. 173 The platinum wires are sterilized by holding them in the flame, till incandescent. The end of the glass rod should always be thoroughly heated, and, if any organic matter is present, such as gelatin, it should be burned off. The wires should be kept in a conical test-glass (Pig. 43), in the bottom of which is some cotton.. Fig. 29. The inoculation of a single tube. Place three of the sterilized, gelatin tubes in a water- bath which has been warmed to about 30-35°. When the gelatin melts, the tubes are ready for inoc
RM2AFN13P–. Applied bacteriology for nurses. but thoroughly over the sur-face of the blood-serum without breaking its surface. Now replacethe swab in its own tube, plug both tubes, and place them in thebox provided by the health officials. This is to be sent to the bac-teriologic expert. In laryngeal diphtheria the swab is to be passedfar back and rubbed freely against the mucous membrane of thepharynx and tonsils (Anders). We have already said that the diphtheria bacillus whengrown in broth produces a strong poison. In fact, thediphtheria bacilli produce all their evil effects through thispoison. The b
RMPG42NP–. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. 14^ DIVISION II.—COURSE OF DEVELOPMENT OF FUNGI. are several oospheres present the tubes often grow from one to another, and even form branches which grow up to and past different oospheres, and sometimes even pierce through the wall of the oogonium and pass outside it; but they always remain closed and die in the course of 1-2 days while the oospheres are maturing. The short tubes of Aphanomyces scaber are the only ones which I have examined which never showed this l
RM2AWX1E5–Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . t of chloroform isadded—^^enough to form abouta 2 mm. layer on the bot-tom. If the chloroform isnot allowed to evaporate,the serum remains sterUefor a long time. Whenneeded for use, test-tubes arefilled and placed in a water-bath at 50° C. until all chlo-roform has been driven off(determined by absence ofcharacteristic odor); the se-rum is then solidified andsterilized as in the ordinaryway, or may be used in afluid state. Htiman Blood-sertun.— Blood-serum derived from
RMPFTPE5–. Agricultural bacteriology; a study of the relation of germ life to the farm, with laboratory experiments for students, microorganisms of soil, fertilizers, sewage, water, dairy products, miscellaneous farm products and of diseases of animals and plants. Bacteriology, Agricultural. 342 LABORATORY WORK No. 18. Fennentation Tubes.—Prepare 200 c.c. of bouillon as described in No. 2, adding to it 2 grams lactose and adjust the reaction to the neutral point. To a second lot of bouillon add the same amount of dextrose and to a third lot the same amount of saccharose. After dissolving and filtering
RM2AWWFAA–Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . (Fig, 54). On hlood-coagulum(see p. 72) the growth is usually grayand the margins of the culture crenated.Often a diagnosis can be made in fourhours if the serum-tubes are kept in theoven at 37° C. In milk, abundantgrowth, without curdling. Bouillon.—^In bouillon an abundantgrowth takes place, and this medium isused to obtain the toxins. Staining.—Is positive by Gramsmethod. Stained best with LoiSers al-kaline methylene-blue. Neissers doublestain (see p. 52) shows granu
RMPG2R3N–. Laboratory work in bacteriology. Bacteriology. BOUILLON, AGAE AND MILK. 23T While the filter is still hot, and the pump active, the well sedimented agar (p. 236) is slowly poured on the cen- ter of the filter. The liquid passes through as rapidly as it strikes the surface. The impurities are brought last upon the filter. If the filtrate is not perfectly clear, it can be passed through the filter a second or a third time. The operation of filtration requires but a few minutes. The resulting filtrate, when filled into tubes and sterilized, is usually wholly free from a de- posit, and is perfec
RM2AJ799X–Journal of bacteriology . Furnishing the requirements ofBacteriologists and Pathologists is an important function of our business, for whichwe maintain a special department and a large variedstock, assuring patrons of Prompt and Intelligent Service JII addition to the usual equipment, Microscopes—Incubators—GeneralGlassware, etc. Prompt shipment from stock is offered of AMERICAN MADEPETRI DISHES 100 X 10 mm, 100 x 15 mm and other sizes BACTERIOLOGICAL TEST TUBES Heavy wall, with and without lip. All sizes GRADUATED PIPETTESPYREX GLASSWARE Beakers, Flasks, etc., many forms and sizes Etc. Etc. o
RMPFTPE9–. Agricultural bacteriology; a study of the relation of germ life to the farm, with laboratory experiments for students, microorganisms of soil, fertilizers, sewage, water, dairy products, miscellaneous farm products and of diseases of animals and plants. Bacteriology, Agricultural. Fig. 58.-—Showing method of hardening agar slants. Place 10 c.c. of this agar medium into each of 25 test-tubes and plug with cotton. In filling these tubes use either a 10 c.c. pipette or a funnel, taking care not to allow the agar to touch the sides of the tubes where the cotton is to be inserted. {e) Place the t
RM2AFN6E9–. Applied bacteriology for nurses. Fig. 10.—Method of holding tubes during inoculation (McFarland). culture, we have at hand the tube containing our originalpure culture and a tube of the sterile medium, e. g.,sterile broth, closed at the top with a cotton plug. Allwe need to do is to transfer a little loopful of culture by CULTIVATIOxX OF BACTERIA 29 means of the platinum wire loop to the tube of sterilebroth. In order to avoid carrying along other germs,however, we first hold the wire loop in the flame untilit glows, thus destroying any germs that may have. Fig. 11.—Incubator (Eyre). lodged
RMPG1TJR–. Laboratory work in bacteriology. Bacteriology. TUBING OF MEDIA. 511 drawn up into a sterile bulb pipette (Pig. 61), and then transferred direct to these tubes. The addition of sterile litmus solution to sterile media is preferable to the ordinary method of adding the litmus to the medium before steril- ization. Tubing of Media. In many experiments, as in the disinfection tests pres ently to be described, it is desirable to employ measured .amounts of bouillon. This can be done expeditiously by means of the simple apparatus shown in Pig. 72. The lower end of a 100 c.c. burette is attached to
RM2AJ77D0–Journal of bacteriology . Furnishing the requirements ofBacteriologists and Pathologists is an important function of our business, for whichwe maintain a special department and a large variedstock, assuring patrons of Prompt and Intelligent Service JII addition to the usual equipment, Microscopes—Incubators—GeneralGlassware, etc. Prompt shipment from stock is offered of AMERICAN MADEPETRI DISHES 100 X 10 mm, 100 x 15 mm and other sizes BACTERIOLOGICAL TEST TUBES Heavy wall, with and without lip. All sizes GRADUATED PIPETTESPYREX GLASSWARE Beakers, Flasks, etc., many forms and sizes Etc. Etc. o
RMPG2R59–. Laboratory work in bacteriology. Bacteriology. 164 BACTERIOLOGY. In this laboratory the Koch sterilizer has been entirely sup- planted by the simple sterilizer' shown in Fig. 26. It may be consid- ered as a modification of the former. It consists of the ordinary Hoffmann iron water-bath, which has an internal diameter of 18 cm., and a copper pail (20 X 20 cm.) which is provided with a perforated bottom. Two perforated rings, on the inside, allow the passage of steam, and prevent the cotton of the tubes from resting against the side of the pail.. Please note that these images are extracted fr
RM2AKX9KM–Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . r by an inert gas, usually by hy-drogen. Inoculate the culture medium as above. Place in a larger vesselprovided with entrance and exit tubes and tightly sealed. Pass a stream ofpurified hydrogen through the vessel continuously for several hours, close thestoppers and place the apparatus in the incubator. CLINICAL BACTERIOLOGY. 707 (3) Absorption of the oxygen. This is the more commonly employedmethod. Place the culture tubes on a glass or metal support inside a largertube or bottle. P
RMPG2WX6–. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. a84 DIVISION II.^COVRSE OF DEVELOPMENT OF FUNGI. without a winter's rest. The further development of the sporidia abscised from the promycelium is perfectly known, at least in L. Dianthi, L. Malvacearum, and L. Circaeae; the germ-tubes from the sporidia penetrate at once into the proper host, either through the wall of an epidermal cell, as in L. Malvacearum, or through a stoma, as in L. Dianthi, and develope a mycelium which again produces only teleuto- spores. Aecid
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